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rhil 7  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec rhil 7
    Rhil 7, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil+7/Human+IL-7%2C+premium+grade/pmc13108567-169-12-15
    Average 96 stars, based on 130 article reviews
    rhil 7 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model
    Article Snippet: Bulk transduced T cells were expanded and restimulated using 0.5–1 × 10 6 TCR-transduced T cells, 2 × 10 5 irradiated (2,500 rads) T2 cells pulsed with 1 μg/mL of either HBs183-91 peptide (FLLTRILTI) or HBcore18-27 (FLPSDFFPSV) (Genscript, custom synthesized peptides) and 1.8 × 10 6 irradiated PBMCs as feeders. .. Cells were cultured for about 2 weeks in AIM-V, 2% human AB serum supplemented with 100 IU/mL of rhIL-2, 10 ng/mL of rhIL-7 (Miltenyi Biotec, 130-095-362), and 10 ng/mL of rhIL-15 (Miltenyi Biotec, 130-095-764). .. In some experiments, CD8 + T cells were enriched through negative selection of transduced T cells using CD4 microbeads (Miltenyi Biotec, 130-045-101) following the manufacturer’s instructions.

    Article Title: Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model.
    Article Snippet: Bulk transduced T cells were expanded and restimulated using 0.5–1 × 106 TCR-transduced T cells, 2 × 105 irradiated (2,500 rads) T2 cells pulsed with 1 μg/mL of either HBs183-91 peptide (FLLTRILTI) or HBcore18-27 (FLPSDFFPSV) (Genscript, custom synthesized peptides) and 1.8 × 106 irradiated PBMCs as feeders. .. Cells were cultured for about 2 weeks in AIM-V, 2% human AB serum supplemented with 100 IU/mL of rhIL-2, 10 ng/mL of rhIL-7 (Miltenyi Biotec, 130-095-362), and 10 ng/mL of rhIL-15 (Miltenyi Biotec, 130-095-764). .. In some experiments, CD8+ T cells were enriched through negative selection of transduced T cells using CD4 microbeads (Miltenyi Biotec, 130-045-101) following the manufacturer’s instructions.

    Quantitation Assay:

    Article Title: Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1.
    Article Snippet: Total CD4+ T cells from HIV-1-infected MACS participants were treated with Dynabeads® Human T-Activator CD3/CD28 (Life Technologies Cat# 11131D) and implemented as a positive control in LR experiments. .. The cytokines rhIL-2 (Proleukin®, 100 U/mL; Prometheus Laboratories, Inc. Cat# NDC65483-116-07) and rhIL-7 (1 μg/mL; Miltenyi Biotec Cat# 130- 095-361) were added to the cultures on day 4, and culture supernatants were harvested on day 7 for quantitation of HIV-1 RNA. ..

    Article Title: Compositions and methods for treating HIV infection and reversing HIV latency
    Article Snippet: Total CD4+ T cells from HIV-1-infected MACS participants were treated with DYNABEADS® Human T-Activator CD3/CD28 (Life Technologies Cat #11131D) and implemented as a positive control in LR experiments. .. The cytokines rhIL-2 (PROLEUKIN®, 100 U/mL; Prometheus Laboratories, Inc. Cat #NDC65483-116-07) and rhIL-7 (1 μg/mL; Miltenyi Biotec Cat #130-095-361) were added to the cultures on day 4, and culture supernatants were harvested on day 7 for quantitation of HIV-1 RNA. ..

    Article Title: Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1
    Article Snippet: Total CD4 + T cells from HIV-1-infected MACS participants were treated with Dynabeads® Human T-Activator CD3/CD28 (Life Technologies Cat# 11131D) and implemented as a positive control in LR experiments. .. The cytokines rhIL-2 (Proleukin®, 100 U/mL; Prometheus Laboratories, Inc. Cat# NDC65483-116-07) and rhIL-7 (1 μg/mL; Miltenyi Biotec Cat# 130-095-361) were added to the cultures on day 4, and culture supernatants were harvested on day 7 for quantitation of HIV-1 RNA. ..

    Concentration Assay:

    Article Title: Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens
    Article Snippet: Ags added on d1 of culture (prior to R848 and LPS) included MUC1-, HER2/neu- and CMVpp65-derived synthetic peptides added at an optimized final concentration of 50 μg/ml when pulsed singly, or 10 μg/ml each when multiple peptides were pulsed as a cocktail (see Peptide synthesis below); clinical grade Candida albicans extract (CAN, Hollister Stier, Spokane WA, #5053, at a 10% v:v optimized final concentration); and recombinant HER2/neu intracellular domain protein (HER2-ICD, SignalChem, Richmond,BC #E27-11G at an optimized final concentration of 50 μg/ml). .. Beginning with culture Step 2 (d2 of overall culture), rhIL-7 (Miltenyi Biotec premium grade, Auburn CA, #130-095-364) was added at an optimized final concentration of 50 ng/ml to fresh medium added at culture splits. .. Other agents tested in Step 2 but absent from the optimized formulation included rhIL-2 (aldesleukin, Prometheus Labs, San Diego, CA, final concentration 24 IU/ml); rhIL-15 (Peprotech #200-15, final concentration 5 ng/ml) and rhIL-21 (Peprotech #200-21, final concentration 3 ng/ml).

    In Vivo:

    Article Title: Targeting PD-1 + T cells with chimeric antigen receptors to reduce the HIV reservoir
    Article Snippet: Medium was supplemented with recombinant human IL-2 (30 IU/ml; rhIL-2, Miltenyi Biotec, Bergisch-Gladbach, Germany, catalog no. 130-097-746) for T cells or CD4 + T cells, 100 IU/ml for CD8 + T cells when expanded alone. .. For the in vivo experiments, the T cell medium was enriched with rhIL-7 (5 ng/ml; Miltenyi Biotec, Bergisch-Gladbach, Germany, catalog no. 130-095-362), rhIL-15 (5 ng/ml; Miltenyi Biotec, Bergisch-Gladbach, Germany, catalog no. 130-095-764), and IL-2 (30 IU/ml). ..



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